The Mycolith project is about developing the bio-fabrication strategy for stabilizing regolith using mycelium, to construct habitat structures, in resource-limited space environments. The project focuses on tackling distinct environmental challenges, such as extreme temperatures and radiation while prioritizing efficient resource utilization. It aims to decrease dependence on external resources, reduce energy usage, and reduce waste production, all to mitigate its influence on lunar and Martian environments while improving long-term sustainability.